NOVEL PREPARATION OF ASYMMETRIC LIPOSOMES WITH INNER AND OUTER LAYER OF DIFFERENT MATERIALS

Asymmetric liposomes whose inner and outer layer with different materi als, which are very useful in liposome and membrane research, have bee n prepared layer by layer from reverse micelles. In general, the diame ters of the liposomes ranged from 30 nm to 100 nm. Fluorescein quenchi ng experiment showed that asymmetric liposomes with inner layer being lipid and outer single-chain [(bpy)(2)Ru(diazafluorenone)(CH2)(15)CH3] (PF6)(2) had been success-fully prepared. The half-life of flip-flop of bilayer is estimated for 17 days. The method present is of signific ance in liposome and membrane study.