SELECTIVE MODULATION OF CELL-ADHESION MOLECULES ON LYMPHOCYTES BY BROMELAIN PROTEASE-5

Human peripheral blood mononuclear cells from healthy donors were trea ted ex vivo with the proteolytic enzyme bromelain and studied by flow cytometry. Bromelain-treated lymphocytes exhibited 60-90% reduced cell surface staining for CD44 and CD62-L molecules. While the staining fo r molecules CD16, CD56 and CD49d was unaffected, a moderate increase ( 10-40%) in expression of the beta(2)-integrins CD11a-c was seen. This selective modulation of cell adhesion molecules (CAM) was seen on T ce lls and NK cells, as well. The selective modulation of CAM may help ex plain some of the clinical effects observed after bromelain treatment in patients suffering from chronic inflammatory disease, HIV and cance r.