A. Mas et al., EPINEPHRINE-INDUCED REDUCTION IN INSULIN-RECEPTOR MESSENGER-RNA LEVELAND STABILITY IN U-937 HUMAN PROMONOCYTIC CELLS, Hormone and Metabolic Research, 30(3), 1998, pp. 118-122
The administration of 10(-5) M epinephrine transiently decreased insul
in receptor (IR) mRNA levels in U-937 human promonocytic cells, which
reached their minimum value after 24 hours. Such a decrease seems to b
e due, at least in part, to a reduction in transcript stability, since
the IR mRNA half-life was observed to decline from approximately 4 h
in untreated cells to 3 h in epinephrine-treated cells. Computer inspe
ction of the sequence of the 3' untranslated region of the human IR mR
NA showed nine AUUUA pentamers and three U-rich regions. These domains
could be targets for a RNA-binding protein induced by treatment with
epinephrine producing a destabilization of IR mRNA in U-937 cells.