IS MTS1 (S100A4) GENE INVOLVED IN THE METASTATIC PROCESS MODULATED BYGAMMA-INTERFERON

This study investigates the involvement of mts1 (S100A4) in the metast atic process. We have evaluated the levels of mts1 gene in murine canc er cells following modulation of the metastatic ability. We report her e that modulation of the expression of mts1 gene in cells derived from a breast murine adenocarcinoma (TS/A) did not correlate with metastat ic ability either when pretreated in vitro with gamma-interferon (gamm a-IFN) or following transfection with gamma-IFN gene. In another serie s of experiments we treated the cell lines B16-A and B78H1, both deriv ed from B16 melanoma, with gamma-IFN. An increased expression of mts1 was found only in B16-A, but not B78H1 correlated with a strong increa se in metastatic activity of B16-A clone upon gamma-IFN treatment.