ASSOCIATION OF THE AP-3 ADAPTER COMPLEX WITH CLATHRIN

Citation
Ec. Dellangelica et al., ASSOCIATION OF THE AP-3 ADAPTER COMPLEX WITH CLATHRIN, Science, 280(5362), 1998, pp. 431-434
Citations number
27
Categorie Soggetti
Multidisciplinary Sciences
Journal title
ISSN journal
00368075
Volume
280
Issue
5362
Year of publication
1998
Pages
431 - 434
Database
ISI
SICI code
0036-8075(1998)280:5362<431:AOTAAC>2.0.ZU;2-B
Abstract
A heterotetrameric complex termed AP-3 is involved in signal-mediated protein sorting to endosomal-lysosomal organelles. AP-3 has been propo sed to be a component of a nonclathrin coat. In vitro binding assays s howed that mammalian AP-3 did associate with clathrin by interaction o f the appendage domain of its beta 3 subunit with the amino-terminal d omain of the clathrin heavy chain. The beta 3 appendage domain contain ed a conserved consensus motif for clathrin binding. AP-3 colocalized with clathrin in cells as observed by immunofluorescence and immunoele ctron microscopy. Thus, AP-3 function in protein sorting may depend on clathrin.