HYDROPEROXIDASE ACTIVITY OF SOYBEAN LIPOXYGENASE IN REVERSE MICELLES

The rate of aminopyrine oxidation in the presence of H2O2 by the hydro peroxidase activity of soybean lipoxygenase was studied as a function of micelle size (omega(o),) and water content (theta) in sodium diocty l sulfosuccinate (AOT) reverse micelles in isooctane. When the micelle size was changed at a fixed water content a saturation activity profi le was obtained, but when both micelle size and water content were cha nged at the same time, a bell-shaped profile was observed. These resul ts indicate that only one micellar parameter (omega(o) or theta) has t o be changed at a time in order to avoid artefactual enzymatic behavio ur. (C) 1998 Elsevier Science B.V. All rights reserved.