OPTIMIZATION OF CELLULAR ELISA FOR ASSAY OF SURFACE-ANTIGENS ON HUMANSYNOVIOCYTES

The cellular enzyme-linked immunosorbent assay (CELISA) permits assay of cell surface antigens on intact fixed cells. Using a monolayer of c ells cells as the solid phase, the CELISA offers an inexpensive altern ative to Slow cytometry. In addition, this protocol has the decided ad vantages of miniaturization (small numbers of cells) and ease of repli cation (the 96-well cluster plate format). In efforts to optimize CELI SA for detecting surface antigens on human fibroblastlike synoviocytes , the authors found that cell number, serum proteins, choice of cultur e plate, pipetting technique and fixatives may all impact the results of the CELISA.