T. Griffiths et al., THE PERFECT(TM) LIPID OPTIMIZER KIT FOR MAXIMIZING LIPID-MEDIATED TRANSFECTION OF EUKARYOTIC CELLS, BioTechniques, 22(5), 1997, pp. 982-987
The transfection efficiencies of a panel of eight uniquely different l
ipid reagents has been evaluated with two other commercially available
lipids for use in transfecting a diversity of eukaryotic cell lines.
The PerFect(TM) lipids are available individually or together in an op
timization panel format that call be tested in any given cell line, en
abling one to evaluate the optimal lipid for transfecting each individ
ual cell line. Our results demonstrate that no single lipid is optimal
for plasmid transfection over a broad range of cell types, thus empha
sizing the need for multiple unique lipid reagents and a simple format
for testing their transfection efficiency on a given cell type.