THE PERFECT(TM) LIPID OPTIMIZER KIT FOR MAXIMIZING LIPID-MEDIATED TRANSFECTION OF EUKARYOTIC CELLS

Citation
T. Griffiths et al., THE PERFECT(TM) LIPID OPTIMIZER KIT FOR MAXIMIZING LIPID-MEDIATED TRANSFECTION OF EUKARYOTIC CELLS, BioTechniques, 22(5), 1997, pp. 982-987
Citations number
7
Categorie Soggetti
Biochemical Research Methods
Journal title
ISSN journal
07366205
Volume
22
Issue
5
Year of publication
1997
Pages
982 - 987
Database
ISI
SICI code
0736-6205(1997)22:5<982:TPLOKF>2.0.ZU;2-0
Abstract
The transfection efficiencies of a panel of eight uniquely different l ipid reagents has been evaluated with two other commercially available lipids for use in transfecting a diversity of eukaryotic cell lines. The PerFect(TM) lipids are available individually or together in an op timization panel format that call be tested in any given cell line, en abling one to evaluate the optimal lipid for transfecting each individ ual cell line. Our results demonstrate that no single lipid is optimal for plasmid transfection over a broad range of cell types, thus empha sizing the need for multiple unique lipid reagents and a simple format for testing their transfection efficiency on a given cell type.