FLAVODOXIN AS AN ELECTRONIC DONOR IN PHOTOSYNTHETIC INORGANIC NITROGEN ASSIMILATION BY IRON-DEFICIENT CHLORELLA-FUSCA CELLS

Authors
VIGARA AJ INDA LA VEGA JM GOMEZMORENO C PELEATO ML
Citation
Aj. Vigara et al., FLAVODOXIN AS AN ELECTRONIC DONOR IN PHOTOSYNTHETIC INORGANIC NITROGEN ASSIMILATION BY IRON-DEFICIENT CHLORELLA-FUSCA CELLS, Photochemistry and photobiology, 67(4), 1998, pp. 446-449
Citations number
32
Categorie Soggetti
Biophysics,Biology
Journal title
Photochemistry and photobiology → ACNP
ISSN journal
00318655
Volume
67
Issue
4
Year of publication
1998
Pages
446 - 449
Database
ISI
SICI code
0031-8655(1998)67:4<446:FAAEDI>2.0.ZU;2-T
Abstract
Two ferredoxin-dependent enzymes involved in the photosynthetic assimi lation of nitrogen, nitrite reductase (EC 1.7.7.1) and glutamate synth ase (EC 1.4.7.1) from Chlorella fusca have been shown to use either fe rredoxin or flavodoxin (from the same organism) as electron donor. Nit rite reductase showed K-m values of 13.8 +/- 0.9 mu M for ferredoxin a nd 30.4 +/- 7.8 mu M for flavodoxin. Glutamate synthase exhibited a K- m of 6.6 +/- 1.2 mu M for ferredoxin compared to a calculated K-m of 2 2.4 +/- 6.4 mu M for flavodoxin.