Ma. Heitkamp et Rg. Orth, AEROBIC BIODEGRADATION POTENTIAL OF A CONSTRUCTED BIOZONE FOR LASAGNA(TM) SOIL REMEDIATION TECHNOLOGY, ENVIRONMENTAL ENGINEERING SCIENCE, 14(3), 1997, pp. 153-161
The biodegradation potential of an aerobic microbial process for p-nit
rophenol (PNP) biodegradation was evaluated for Lasagna(TM) soil remed
iation technology. A unique, flow-through test cell was designed to pa
ss controlled levels of PNP through an aerated biozone containing Pseu
domonas putida PNP1 immobilized on a porous nylon biocarrier. Chemical
-loading studies and kinetic analyses determined a pseudo first-order
rate constant of 0.036 min(-1) and a half-life of 19.1 min for PNP in
the biozone, Microbial respiration, measured as resting oxygen uptake
(Ou) and glucose-specific Ou, established the relationships between ce
ll numbers, oxygen consumption, and biodegradation of PNP. These resul
ts provide the fundamental understanding needed to predict performance
of this aerobic bioprocess under optimal conditions and show the aero
bic microbial bioprocess, if properly implemented and supported, has a
high capacity for chemical degradation and should support most applic
ations of Lasagna(TM) soil remediation technology for remediation of P
NP contaminated soils.