MONITORING OF NEW SILICA-BASED REVERSED-PHASE STATIONARY PHASES FOR THE LIQUID-CHROMATOGRAPHIC ANALYSIS OF BASIC PHARMACEUTICALS USING PRINCIPAL COMPONENTS-ANALYSIS

In this study, fourteen commercially available reversed-phase stationa ry phases were compared for the high performance liquid chromatographi c analysis of basic pharmaceutical compounds. The influence of silanol blocking compounds on retention and peak shape was investigated using phosphate buffers at pH values of 3 and 7. Principal components analy sis was used to analyse the data set, enabling an evaluation of the va rious stationary phases. The commercially available stationary phases showed distinct differences in their suitability for the analysis of b asic compounds, whereas addition of N,N-dimethyloctylamine to mobile p hase buffer at pH 3 caused an improvement in the peak shapes.