METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS DETERMINATION OF ORGANIC AND INORGANIC ACIDS BY CAPILLARY ZONE ELECTROPHORESIS

The separation of organic and inorganic acids was performed by capilla ry zone electrophoresis using indirect UV detection. Optimum condition s for the separation of 11 anions were investigated. This method was a pplied for the simultaneous determination of organic and inorganic ani ons in foods and beverage samples. The relative standard deviation (R. S.D.) for the analysis of anions was less than 1% for the migration ti me and 1-4% for the peak area (n=18). The detection limits was from 0. 006 to 1.072 mg/l for the 11 anions. This newly developed method is ra pid, sensitive and quantitative and can be readily applied to real foo d and beverage samples for quantitative analysis.