ENHANCEMENT, BY SUCCINATE ADDITION, OF THE PRODUCTION OF CLONED GLUCOAMYLASE FROM RECOMBINANT YEAST USING A SUC2 PROMOTER

Authors
CHA HJ KIM MH KIM SH YEO JS CHAE HJ YOO YJ
Citation
Hj. Cha et al., ENHANCEMENT, BY SUCCINATE ADDITION, OF THE PRODUCTION OF CLONED GLUCOAMYLASE FROM RECOMBINANT YEAST USING A SUC2 PROMOTER, Process biochemistry, 33(3), 1998, pp. 257-261
Citations number
9
Categorie Soggetti
Engineering, Chemical",Biology,"Biothechnology & Applied Migrobiology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
33
Issue
3
Year of publication
1998
Pages
257 - 261
Database
ISI
SICI code
1359-5113(1998)33:3<257:EBSAOT>2.0.ZU;2-C
Abstract
The effect of succinate on the production of cloned glucoamylase by a recombinant yeast system was investigated. Saccharomyces cerevisiae wa s transformed with the 2 mu-based plasmid YEpSUCSTA containing the SUC 2 promoter, the STA signal sequence, and the STA structural gene. Spec ific glucoamylase expression was 89% higher in a semi-synthetic medium containing succinate than in medium without succinate. This increase in glucoamylase activity in succinate-containing media reflects two ro les of succinate; protection of rapid decrease in pH in the culture br oth and enhancement of glucoamylase biosynthesis. (C) 1998 Elsevier Sc ience Ltd.