PURIFICATION OF PROTEIN-A FROM STAPHYLOCOCCUS-AUREUS BY AFFINITY-CHROMATOGRAPHY USING CROSS-LINKED MACROPOROUS GLYCIDYL POLYMER

A new process for the purification of protein A from Staphylococcus au reus has been developed achieving purity of more than 98% with a recov ery of 30%. The process involves extraction employing a specific buffe r containing Toluene-Triton X-100-EDTA and subsequent purification usi ng macroporous glycidyl copolymer. The process has been successfully u sed to purify protein A of both extracellular and intracellular origin . The process is simple, rapid, inexpensive and efficient when compare d to many other reported processes. (C) 1998 Elsevier Science Ltd.