Mj. Kujau et S. Wolfl, INTRAMOLECULAR DERIVATIZATION OF 2'-AMINO-PYRIMIDINE MODIFIED RNA WITH FUNCTIONAL-GROUPS THAT IS COMPATIBLE WITH REAMPLIFICATION, Nucleic acids research, 26(7), 1998, pp. 1851-1853
To expand the scope of nucleic acid aptamers as a tool for precise mol
ecular recognition, functional groups that are not naturally present i
n nucleic acid molecules are desired. For in vitro selection these new
functional groups must be compatible with the selection process. The
present method allows the introduction of succinimide activated side c
hains at internal amino groups of 2'-amino-pyrimidine-RNA in a combina
torial fashion that is compatible with enzymatic re-amplification.