ATOMIC-FORCE MICROSCOPY EXAMINATION OF TOBACCO MOSAIC-VIRUS AND VIRION RNA

Citation
Yf. Drygin et al., ATOMIC-FORCE MICROSCOPY EXAMINATION OF TOBACCO MOSAIC-VIRUS AND VIRION RNA, FEBS letters, 425(2), 1998, pp. 217-221
Citations number
21
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
ISSN journal
00145793
Volume
425
Issue
2
Year of publication
1998
Pages
217 - 221
Database
ISI
SICI code
0014-5793(1998)425:2<217:AMEOTM>2.0.ZU;2-G
Abstract
Atomic force microscopy (AFM) was applied to study uncoated virus part icles and RNA prepared by stripping of tobacco mosaic virions (TMV) wi th mild alkali or urea and dimethylsulfoxide. We found that AFM is an appropriate method to study ribonucleoprotein and free RNA structures. Images of entire tobacco mosaic virions, partially uncoated TMV parti cles with protruding RNA molecule from one or both ends and individual RNA molecules are presented. (C) 1998 Federation of European Biochemi cal Societies.