HEXOKINASE PII HAS A DOUBLE CYTOSOLIC-NUCLEAR LOCALIZATION IN SACCHAROMYCES-CEREVISIAE

Authors
RANDEZGIL F HERRERO P SANZ P PRIETO JA MORENO F
Citation
F. Randezgil et al., HEXOKINASE PII HAS A DOUBLE CYTOSOLIC-NUCLEAR LOCALIZATION IN SACCHAROMYCES-CEREVISIAE, FEBS letters, 425(3), 1998, pp. 475-478
Citations number
34
Categorie Soggetti
Biology,"Cell Biology",Biophysics
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
425
Issue
3
Year of publication
1998
Pages
475 - 478
Database
ISI
SICI code
0014-5793(1998)425:3<475:HPHADC>2.0.ZU;2-T
Abstract
We describe here that the HXK2 gene product, isoenzyme Pn of hexokinas e, is located in both the nucleus and the cytoplasm of Saccharomyces c erevisiae cells. This conclusion is supported by assays of hexokinase- specific activity in isolated nuclei from wild-type and hxk1lhxk2 doub le mutant strains, by immunoblot experiments using anti-Hxk2 antibodie s and by observation of the fluorescence distribution of a Hsk2-GFP fu sion protein in cells transformed with the HXK2::gfp gene. (C) 1998 Fe deration of European Biochemical Societies.