A NOVEL LIPOPOLYSACCHARIDE INDUCIBLE C-C CHEMOKINE RECEPTOR-RELATED GENE IN MURINE MACROPHAGES

To identify genes induced in activated macrophages, we screened a cDNA Library prepared from the lipopolysaccharide (LPS)-treated cell line, RAW261, using the suppression subtractive hybridization technique. On e of the clones isolated was dramatically induced by LPS in macrophage s. The predicted protein sequence of this gene contains the domain uni que to seven transmembrane receptors, and shows similarity with mouse C-C chemokine receptor 5 (CCR5), Therefore, we designated it LPS induc ible C-C chemokine receptor related gene (L-CCR), Northern blot analys is revealed that L-CCR was specifically expressed in differentiated ma crophages after LPS stimulation. These results show that L-CCR is a no vel C-C chemokine receptor related gene induced by LPS in macrophages and may play an important role in inflammatory responses. (C) 1998 Fed eration of European Biochemical Societies.