EFFECT OF VARIOUS DEPOLARIZING AGENTS ON ENDOGENOUS AMINO-ACID NEUROTRANSMITTER RELEASE IN RAT CORTICAL-NEURONS IN CULTURE

The purpose of this study is to determine whether differences in membr ane potential and/or intracellular Ca2+ increments are implicated in a programmed release of amino acid neurotransmitters (aspartate, glutam ate, glycine and GABA) in cortical neurons in culture. According to ou r results, it is possible to assume that difference in membrane potent ial is not the only signal which starts the amino acid neurotransmitte r release, but there are other necessary conditions at the start of th is amino acid release. One of these conditions could be the increment in intracellular Ca2+, but our results indicate that, in cortical neur ons in culture, the total intracellular Ca2+ increments are not import ant on release levels, but are the stimulating agent which produces th is intracellular Ca2+ increment. From these results we may infer: (1) that in rat cortical neurons there are neurons which contain and relea se glutamate, aspartate, glycine and GABA, (2) that in cortical neuron s the 36.6+/-5.8% of the neurons are GABA-ergic, (3) that the membrane potential and the total intracellular calcium are not only responsibl e for the release of these amino acids but also the depolarizing agent which plays an important role in this release, and (4) that glutamate and aspartate and glutamate and GABA are localized in different vesic ular pools or in different cell neurons. (C) 1998 Elsevier Science Ltd . All rights reserved.