B. Salmon et al., THE HERPES-SIMPLEX VIRUS TYPE-1 U(L)17 GENE ENCODES VIRION TEGUMENT PROTEINS THAT ARE REQUIRED FOR CLEAVAGE AND PACKAGING OF VIRAL-DNA, Journal of virology, 72(5), 1998, pp. 3779-3788
Previous studies have suggested that the U(L)17 gene of herpes simplex
virus type 1 (HSV-1) is essential for virus replication. In this stud
y, viral mutants incorporating either a lacZ expression cassette in pl
ace of 1,490 bp of the 2,109-bp U(L)17 open reading frame [HSV-1(Delta
U(L)17)] or a DNA oligomer containing an in-frame stop codon inserted
778 bp from the 5' end of the U(L)17 open reading frame [HSV-1(U(L)17
-stop)] were plaque purified on engineered cell lines containing the U
(L)17 gene. A virus derived from HSV-1(U(L)17-stop) but containing a r
estored U(L)17 gene was also constructed and was designated HSV-1(U(L)
17-restored). The latter virus formed plaques and cleaved genomic vira
l DNA in a manner indistinguishable from wild-type virus. Neither HSV-
1(Delta U(L)17) nor HSV-1(U(L)17-stop) formed plaques or produced infe
ctious progeny when propagated on noncomplementing Vero cells. Further
more, genomic end-specific restriction fragments were not detected in
DNA purified from noncomplementing cells infected with HSV-1(Delta U(L
)17) or HSV-1(U(L)17-stop), whereas end-specific fragments were readil
y detected when the viruses were propagated on complementing cells. El
ectron micrographs of thin sections of cells infected with HSV-1(Delta
U(L)17) or HSV-1(U(L)17-stop) illustrated that empty capsids accumula
ted in the nuclei of Vero cells, whereas DNA-containing capsids accumu
lated in the nuclei of complementing cells and enveloped virions were
found in the cytoplasm and extracellular space. Additionally, protein
profiles of capsids purified from cells infected with HSV-1(Delta U(L)
17) compared to wild-type virus show no detectable differences. These
data indicate that the U(L)17 gene is essential for virus replication
and is required for cleavage and packaging of viral DNA. To characteri
ze the U(L)17 gene product, an anti-U(L)17 rabbit polyclonal antiserum
was produced. The antiserum reacted strongly with a major protein of
apparent M-r 77,000 and weakly with a protein of apparent M-r 72,000 i
n wild-type infected cell lysates and in virions. Bands of similar siz
es were also detected in electrophoretically separated tegument fracti
ons of virions and light particles and yielded tryptic peptides of mas
ses characteristic of the predicted U(L)17 protein. We therefore concl
ude that the U(L)17 gene products are associated with the virion tegum
ent and note that they are the first tegument-associated proteins show
n to be required for cleavage and packaging of viral DNA.