ANALYSIS OF CHARGED CYCLODEXTRIN DERIVATIVES BY ONLINE CAPILLARY ELECTROPHORESIS IONSPRAY-MASS SPECTROMETRY

On-line capillary electrophoresis-ionspray-mass spectrometry (CE-ISP-M S) was applied to a composition analysis of charged cyclodextrin (CD) derivatives. Quaternary ammonium beta-CD (QA-beta-CD) and carboxymethy lated gamma-CD (CM-gamma-CD), which were used as chiral selectors for enantiomer separations by CE, were analyzed. As for QA-beta-CD, eight components having different degrees of the quaternary ammonium substit ution could be identified based on their mass spectra. The components were detected as either molecular ions or fragment ions. On the other hand, six components of CM-gamma-CD were detected as bivalent molecula r ions. Such composition analyses were difficult using CE with only th e UV detector. CE-MS was found to be a promising technique for a compo sition analysis of mixed compounds.