PRODUCTION OF A HYBRID PROTEIN CONSISTING OF THE N-TERMINAL FRAGMENT OF UROKINASE AND THE C-TERMINAL DOMAIN OF URINARY TRYPSIN-INHIBITOR INESCHERICHIA-COLI

We have constructed a hybrid protein (ATFHI) consisting of an N-termin al fragment from urokinase (ATF) and HI-8, which is the C-terminal dom ain of urinary trypsin inhibitor. The fusion genes for the hybrid prot eins were engineered by PCR and cloned into expression plasmids, Under the control of the toc promoter, fusion genes were efficiently expres sed in Escherichia cell, The hybrid proteins, produced as inclusion bo dies in E. coil, were refolded by a dialysis method and purified by io n-exchange chromatography, ATFHI exhibited bifunctional activity relat ed to antimetastatic effects: the urokinase receptor-binding activity of ATF and the inhibitory activity of HI-8 on plasmin.