RELATIONSHIP BETWEEN 2,5-HEXANEDIONE CONCENTRATIONS IN NERVE, SERUM, AND URINE ALONE OR UNDER CO-TREATMENT WITH DIFFERENT DOSES OF METHYL ETHYL KETONE, ACETONE, AND TOLUENE

To ascertain the relationship among 2,5-hexanedione (2,5-HD) concentra tions in nerve, serum and urine, rats were injected subcutaneously wit h 2.6 mmol/kg 2,5-HD alone, or together with 2.6 or 13.0 mmol/kg of me thyl ethyl ketone, acetone and toluene. 2,5-HD concentrations in sciat ic nerve (NC), serum (SC) and urine (UC) were determined, and the line ar regression between each two of NC, SC, and UC were calculated. Ther e was good correlation between NC and SC, SC and UC in the 2,5-HD alon e group, and good correlation between NC and SC in the co-treated grou ps. Go-treatment solvent had little effect on the relationship between SC and NC. 13.0 mmol/kg cotreated solvent tended to decrease the regr ession coefficients compared with 2.6 mmol/kg co-heated solvent. These results show that SC can be used in estimating NC in the 2,5-HD alone or cotreated groups, and UC can be used in estimating SC in the 2,5-H D alone group.