ENANTIOMER SEPARATION USING BSA AS CHIRAL STATIONARY-PHASE IN AFFINITY OTEC AND OTLC

The protein bovine serum albumin (BSA) was chemically bound to the inn er surface of fused silica capillaries by a gentle coupling procedure in order to form a stable chiral stationary phase. Enantiomer separati on of a number of DNP-amino acids and 3-hydroxy-1,4-benzodiazepines wa s possible in affinity open tubular electrokinetic chromatography (aff inity OTEC) and open tubular liquid chromatography (OTLC) in one instr umental setup. This approach combines the advantages of high efficienc y and small consumption of solvent and chiral selector with the possib ility of UV detection without limitations of protein absorption. (C) 1 998 John Wiley & Sons, Inc.