USE OF AKIS-(2,3-O-DIMETHYL-6-SULFATO)-BETA-CYCLODEXTRIN, A NEW SINGLE-ISOMER CHIRAL RESOLVING AGENT FOR THE SEPARATION OF THE ENANTIOMERS OF NEUTRAL COMPOUNDS BY CAPILLARY ELECTROPHORESIS

The single isomer, fully and permanently charged eptakis-(2,3-dimethyl -6-sulfato)-beta-cyclodextrin was used to study the complexation behav ior of the enantiomers of noncharged analytes in capillary electrophor esis. Separation selectivities were calculated from the measured effec tive mobilities and were used to determine the individual complexation constants by fitting the experimentally obtained selectivity values t o the theoretically derived selectivity expression according to the ch arged resolving agent migration model of capillary electrophoresis. Th ough eptakis-(2,3-dimethyl-6-sulfato)-beta-cyclodextrin complexed very weakly with the neutral analytes studied, good separation selectiviti es were observed. For all neutral analytes, separation selectivity dec reased with increasing concentration of the charged cyclodextrin as lo ng as the analytical concentration of the charged cyclodextrin was muc h greater than that of the analyte. In addition to adequate separation selectivity, good peak resolution required the simultaneous control o f the magnitude of both the effective charge of the analyte and the di mensionless electroosmotic now rate. (C) 1998 John Wiley & Sons, Inc.