REDUCTION OF PLASMA-VOLUME AFTER STORAGE OF PLATELETS IN CP2D

BACKGROUND: There has been concern that further deterioration might oc cur if stored platelets are centrifuged to reduce their volume. Althou gh such centrifugation appears to have minimal effect on platelets in CPDA-1 (osmolarity, 470 mOsm) there is no information on the situation with CP2D (580 mOsm). STUDY DESIGN AND METHODS: Platelet concentrates from CP2D packs were sampled at 1 and 5 days and after centrifugation was used to reduce the plasma volume to 10 mt. The aggregation, hypot onic shock response, morphology, pH, and lactate, glucose, pCO(2) and pO(2), levels were assessed, and values were compared to those seen wi th CPDA-1. In addition, blood was collected from the same donors into both CP2D and standard sodium citrate anticoagulant in an anticoagulan t-to-blood ratio of 1:8 and the aggregation response of the fresh plat elets was measured. RESULTS: Collection of blood into CP2D results in an immediate reduction of the platelet aggregation response when compa red to that found after collection of blood into sodium citrate or CPD A-1. Aggregation is further decreased after storage; however, these ch anges and those for hypotonic shock, pH,, lactate, glucose, and pCO(2) , are similar to those seen for CPDA-1. Additional centrifugation did not cause further change. CONCLUSION: Platelets stored in CP2D have re duced in vitro function after 5 days of storage, but subsequent centri fugation to reduce the plasma volume does not further alter these plat elets.