EFFECT OF SUGARS ON POSTTHAW SEMINAL CHARACTERISTICS OF HF SEMEN IN TRIS BUFFER IN ABSENCE AND PRESENCE OF GLYCEROL

Post-thaw motility (PTM) and post-thaw aging motility (PTAM) were sign ificantly (P < 0.01) higher in tris containing glycerol than that with out glycerol, almost with all the sugars. In the absence of glycerol p ost-thaw incubation motility (PTIM) and PTAM did not differ from each other due to different sugars. But in presence of glycerol PTM and PTI M were significantly (P < 0.01) higher with raffinose than that with o ther sugars. Higher number of sperm survived freezing stress in presen ce of glycerol and per cent live sperm differed significantly (P < 0.0 5) due to different sugars. Glycerol and sugars combinedly exerted ben eficial effect on acrosomal maintenance also. Higher number of per cen t normal acrosome was recorded in presence of glycerol and sugars exce pt 2.0% sucrose and 2.0% table sugar (the higher molecular weight suga rs).