Pu. Le et al., INCREASED BETA-ACTIN EXPRESSION IN AN INVASIVE MOLONEY SARCOMA VIRUS-TRANSFORMED MDCK CELL VARIANT CONCENTRATES TO THE TIPS OF MULTIPLE PSEUDOPODIA, Cancer research, 58(8), 1998, pp. 1631-1635
An invasive variant of Moloney sarcoma virus-transformed MDCK cells (M
SV-MDCK-INV), which was isolated by the repeated selection of cells th
at successfully traversed a Matrigel-coated filter, exhibits increased
motile ability and presents an elongated cell shape and numerous pseu
dopodia, Although stress fibers are present in both MDCK and MSV-MDCK
cells, MSV-MDCK-INV cells contain no stress fibers and exhibit a dense
concentration of actin at the tips of pseudopodia, Relative to both M
DCK and MSV-MDCK cells, the MSV-MDCK-INV cells exhibit increased expre
ssion of beta-actin and redistribution of beta-actin to the tips of ps
eudopodia. These actin concentrations are enriched in both F-and G-act
in and, thus, represent dynamic regions of actin cytoskeleton remodeli
ng. The acquisition of invasive properties by epithelial transformants
is, therefore, associated with the increased expression of beta-actin
and its concentration in actin-rich domains, which may drive pseudopo
dial extension and facilitate tumor cell invasion.