A NOVEL HUMAN GLASSY-CELL CARCINOMA CELL-LINE PRODUCING IL-6 AND IL-8FROM UTERINE CERVIX

A novel human cell line, TOM-2. was established from a rare uterine ce rvical cancer, glassy cell carcinoma (GCC). TOM-2 is the second establ ished GCC cell line so far reported. The cells were intermediately or poorly differentiated with dysplastic nuclei and polygonal shape and s ecreted two tumor markers and cytokines, i.e., CA-125 and SCC, interle ukin (IL)-1 alpha, -6, and -8, and TNF-alpha. Growth of TOM-2 was so s trongly dependent on population density that it was not possible to de termine the plating efficiency. In mass culture, the following charact eristics were observed: doubling time, 83 h; mode of chromosome number , 79; human papillomavirus type 18 DNA, detectable; tumorigenicity, ea sily transplantable into subcutis of nude mice: chemosensitivity in vi tro, considerably sensitive to Cisplatin and 5-FU but not to 9 other a ntineoplastic agents. This novel cell line will be useful for developi ng new therapeutic strategies for the rare cancer, GCC.