N. Yonemitsu et al., INFLUENCE OF EXTRACELLULAR-MATRIX ON THE LIPOGENESIS OF CULTURED WHITE FAT-CELLS, Acta histochemica et cytochemica, 31(1), 1998, pp. 9-15
Collagenase digests from subcutaneous fat tissue of new born rats were
cultured on different types of collagen gel containing 50% type I col
lagen, on fibronectin (Fn) or on laminin (Lm). On day 6, 17% of cells
on a plastic substratum differentiated and had multilocular or unilocu
lar cytoplasmic lipid droplets (CLDs). Cells on each type of collagen
and on Lm had more CLDs than those on a plastic substratum. The extent
of lipogenesis showed the following decreasing order: cells on Lm (80
%), on type IV+I collagen (70%), on type I collagen (52%), on type III
+I collagen (36%), on type II+I collagen (32%). On day 14, most cells
on Lm became unilocular fat cells. Cells on Fn showed delipidation and
showed decreased reactivity to insulin stimulation. Immunohistochemic
ally differentiated fat cells were positively stained with antilaminin
or anti-type IV collagen antibodies. These results provide evidence t
hat Lm and type IV collagen promote fat cell differentiation, whereas
Fn facilitates dedifferentiation into the fibroblast-like fat cell.