THE PROTEIN-KINASE0N (PKN) GENE PRKCL1 PRKCL1 MAPS TO HUMAN-CHROMOSOME 19P12-P13.1 AND MOUSE-CHROMOSOME-8 WITH CLOSE LINKAGE TO THE MYODYSTROPHY (MYD) MUTATION/

Protein kinase N (PKN) is a fatty acid- and Rho-activated serine/threo nine protein kinase involved in the regulation of cell motility by ass ociation with cytoskeletal components such as neurofilament and cu-act inin. We determined the chromosomal location of the human PKN gene PRK CL1 by fluorescence in situ hybridization and by radiation hybrid mapp ing. The corresponding mouse gene Prkcl1 was mapped by segregation ana lysis. We found by FISH that PRKCL1 is localized to chromosome 19p12-p 13.1 and, more precisely, by radiation hybrid mapping, about 11 cR fro m EST WI-6344 in subband 19p12. Prkcl1 maps to mouse chromosome 8 betw een D8Mit6 and junb. This region of mouse Chr 8 shows a scrambled synt enic conservation to human chromosomes 4q, 8p, and 19p. As the mouse m utation myodystrophy myd has been mapped to the same region, Prkcl1 is a candidate gene for myd. (C) 1998 Academic Press.