MECHANISM OF INITIATION OF YEAST MITOCHONDRIAL-DNA REPLICATION - ROLEOF RNA-POLYMERASE

Authors
DSOUZA S PASUPATHY K
Citation
S. Dsouza et K. Pasupathy, MECHANISM OF INITIATION OF YEAST MITOCHONDRIAL-DNA REPLICATION - ROLEOF RNA-POLYMERASE, Journal of Biosciences, 22(2), 1997, pp. 149-159
Citations number
24
Categorie Soggetti
Biology
Journal title
Journal of Biosciences → ACNP
ISSN journal
02505991
Volume
22
Issue
2
Year of publication
1997
Pages
149 - 159
Database
ISI
SICI code
0250-5991(1997)22:2<149:MOIOYM>2.0.ZU;2-Z
Abstract
Yeast mitochondrial RNA polymerase was purified and resolved into 2 di stinct fractions, Peak A was found to be nonspecific and exhibited cha racteristics of the core polymerase, whereas peak B exhibited characte ristics of the holoenzyme. In vitro replication assays were carried ou t, using the peak B enzyme, the cloned ori sequences and other DNA tem plates. It was found that ori 2 was the most efficient template for RN A polymerase primed DNA synthesis, as compared to all the other templa tes studied.