C. Charpin et al., ELAM SELECTIN EXPRESSION IN BREAST CARCINOMAS DETECTED BY AUTOMATED AND QUANTITATIVE IMMUNOHISTOCHEMICAL ASSAYS, International journal of oncology, 12(5), 1998, pp. 1041-1048
ELAM is an E-Selectin adhesion molecule involved in the inflammatory p
rocess but it is also thought to potentially participate in the develo
pment of blood borne metastases, by facilitating tumour cell adhesion
to vessels wall. ELAM expression in rumours was immunohistochemically
investigated in 203 breast carcinomas. Frozen tissue sections were pro
bed with monoclonal anti ELAM (Clone 1.2B6) using automated and quanti
tative immunoperoxidase systems. A positive anti-ELAM immunoreaction w
as observed in 113 tumours (57%). The mean surface of positive tumours
varied from 3% to 50% (mean = 11.75%, SD = 8.7) and was correlated wi
th histoprognostic indicators and tumour expression of various antigen
s detected according to the same method as ELAM. The results showed th
at ELAM immunoexpression was independent of the tumour size, grade and
type and of the nodal status but significantly increased parallel to
patients' age (p<0.01). ELAM expression was independent of Ki-67/MIB1,
anti-P53 and anti-Bcl2, anti-CD44v, anti-c-erbB-2, anti-CD31, anti-RE
/RP, anti-PS2, and anti-VLA, immunoreactions. But ELAM expression corr
elated with that of the VCAM vascular cell adhesion molecule (p=0.0004
), VLA, (p<0.0001), P-glycoprotein (p=0.025), and of Cathepsin D to a
lower degree (p=0.06) and inversely correlated with E-cadherin (p=0.03
). The results suggest that endothelial cell activation is independent
of tumour cell proliferative activity and of stromal angiogenesis and
that the precise role and regulation of ELAM in rumours remains to be
elucidated. Also the clinical relevance of ELAM immunohistochemical e
xpression requires further investigation and correlation with patients
' follow-up.