CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF INSULIN T3R3 STATES CREATED IN DIFFERENT CONDITIONS

In the presence of a series of small organic molecules including 1, 4- dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn-->Ser a nd (A21 Asn-->Ser, B27 Thr-->Arg) human insulin have been crystallized in both Zn2+-containing and Zn2+-free system. Preliminary crystallogr aphic analyses show that they ail belong to T3R3 structural type. The results indicate thar, except phenolic derivatives and lyotropic anion s reported before, some other appropriate organic molecules can also i nduce the T3R3 conformation transition of insulin.