TRANSCRIPTION OF MULTIPLE CELL-WALL PROTEIN-ENCODING GENES IN SACCHAROMYCES-CEREVISIAE IS DIFFERENTIALLY REGULATED DURING THE CELL-CYCLE

The yeast cell wall consists of an internal skeletal layer and an outs ide protein layer. The synthesis of both beta-1,3-glucan and chitin, w hich together form the cell wall skeleton, is cell cycle-regulated. We show here that the expression of five cell wall protein-encoding gene s (CWP1, CWP2, SED1, TIP1 and TIR1) is also cell cycle-regulated. TIP1 is expressed in G1 phase, CWP1, CWP2 and TIR1 are expressed in S/G2 p hase, and SED1 in M phase. The data suggest that these proteins fulfil distinct functions in the cell wall. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.