Sj. Feng et Ptk. Woo, CHARACTERIZATION OF A 200 KDA GLYCOPROTEIN (CS-GP200) ON THE PATHOGENIC PISCINE HEMOFLAGELLATE CRYPTOBIA-SALMOSITICA, Diseases of aquatic organisms, 32(1), 1998, pp. 41-48
The 200 kDa antigenic doublet of the pathogenic haemoflagellate Crypto
bia salmositica Katz, 1951, was detected using a monoclonal antibody (
MAb-001) in 1-dimensional SDS-PAGE. This antigenic doublet is a glycop
rotein since it was susceptible to both protease K and to sodium m-per
iodate oxidation and is designated Cs-gp200. It has a PI (isoelectric
point) value of about 5.5 (using 2-dimensional gel electrophoresis). I
t migrated faster under reducing conditions than under nonreducing con
ditions and was partitioned into the aqueous phase in Triton X-114 pha
se separation. It is a secretory-excretory product since it was detect
ed in a non-protein culture medium with C. salmositica. These results
suggest that the Cs-gp200 is a glycoprotein consisting of carbohydrate
determinants and conformational polypeptide with internal disulphide
bonds. It is a hydrophilic antigen, is a secretory product of the para
site, and plays a important role in antibody elicitation in immunized
fish.