IL-10 PRODUCTION IN COMMON VARIABLE IMMUNODEFICIENCY

Common variable immunodeficiency, (CVI) is a heterogeneous primary imm unodeficiency disease in which there are T and B cell defects. Since I L-10 in conjunction with anti-CD40 promotes secretion of IgG, IgA, and IgM by CVI B cells, these studies were performed to investigate IL-10 production in CVI. Mitogen or anti-CD3 stimulated CVI peripheral bloo d mononuclear cells, or isolated T cells produced an insignificant amo unt of IL-10 over background levels. CVI monocyte IL-10 production was substantial and greater than that of normal controls. Anti-IL-10-neut ralizing antibody strongly enhanced CVI T cell proliferative responses to PHA, but only to an insignificant extent, soluble antigens. IL-2 p lus anti-IL-10 enhanced CVI proliferative responses to antigens signif icantly more over baseline than for cells of similarly tested normal c ontrols. These data suggest that CVI T cell secretion of IL-10 is defi cient, but that monocyte-derived IL-10, plus a relative lack of IL-2 p roduction, could contribute to the defects of cell proliferation in th is disorder. (C) 1998 Academic Press.