GLYCINE68 TO HISTIDINE73 HAS AN IMPORTANT ROLE IN THE FUNCTION OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA

Authors
CEN B JIN WX WU HH LU J DONG XY XU XX
Citation
B. Cen et al., GLYCINE68 TO HISTIDINE73 HAS AN IMPORTANT ROLE IN THE FUNCTION OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA, Biochemistry and molecular biology international, 43(1), 1997, pp. 47-52
Citations number
18
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
43
Issue
1
Year of publication
1997
Pages
47 - 52
Database
ISI
SICI code
1039-9712(1997)43:1<47:GTHHAI>2.0.ZU;2-M
Abstract
Mutant human tumor necrosis factor alpha(hTNF alpha) genes have been c onstructed by in vitro mutagenesis and expressed in Escherichia coli. A deletion involving Gly68 to His73 in hTNF alpha remarkably decreased the solubility and biological activity of hTNF alpha. From the above and results of a molecular dynamics simulation it is proposed that the region of Gly68 to His73 in hTNF alpha has an important role in the m aintenance of the 3-D structure and modulation of the biological activ ity of hTNF alpha.