Kh. Lee et al., ATTENUATION OF RENOMEDULLARY PHOSPHOLIPASE-C ISOZYME, PLC-DELTA-1, INSPONTANEOUSLY HYPERTENSIVE RATS, Biochemistry and molecular biology international, 43(4), 1997, pp. 741-747
The distributional patterns of PLC isozymes within the kidney were inv
estigated using spontaneously hypertensive rats (SHRs) and normotensiv
e Wistar-Kyoto (WKY) rats at 4 and 12 weeks of age. PLC-beta 1, PLC-be
ta 3 and PLC-delta 1 quantified by Western blot analysis, were present
in the highest concentrations in the inner medulla of fats at both 4
and 12 weeks of age. On the other hand, PLC-beta 4, PLC-gamma 1 and PL
C-gamma 2 were distributed almost equally among the regions for the ra
ts of both ages. When compared with WKY rats at 12 weeks of age, the a
mounts of PLC-beta 1, PLC-beta 3, PLC-gamma 1, PLC-gamma 2, and PLC-de
lta 1 in the inner medulla of SHRs were significantly lower, and the a
mount of PLC-delta 1 in the inner stripe of the outer medulla was also
significantly lower. Even at the prehypertensive stage at 4 weeks of
age, the inner medullary concentration of PLC-delta 1 was significantl
y lower in SHRs than WKY rats. These results suggest that PLC-delta 1
would play an important role in the development of hypertension.