IN-VITRO DIGESTIVE FLUID EXTRACTION AS A MEASURE OF THE BIOAVAILABILITY OF SEDIMENT-ASSOCIATED POLYCYCLIC AROMATIC-HYDROCARBONS - SOURCES OF VARIATION AND IMPLICATIONS FOR PARTITIONING MODELS
Dp. Weston et Lm. Mayer, IN-VITRO DIGESTIVE FLUID EXTRACTION AS A MEASURE OF THE BIOAVAILABILITY OF SEDIMENT-ASSOCIATED POLYCYCLIC AROMATIC-HYDROCARBONS - SOURCES OF VARIATION AND IMPLICATIONS FOR PARTITIONING MODELS, Environmental toxicology and chemistry, 17(5), 1998, pp. 820-829
In vitro extraction of contaminated sediments using the digestive flui
d of a deposit-feeding polychaete has recently been proposed to study
contaminant bioaccumulation mechanisms and perhaps to better quantify
the bioavailable contaminant fraction. This approach was evaluated usi
ng digestive fluid from the polychaete Arenicola brasiliensis and six
marine sediments containing both spiked radiolabeled polycyclic aromat
ic hydrocarbons (PAHs) anti in situ-contaminated unlabeled PAHs. The p
roportion of total contaminant extracted by digestive fluid from each
sediment varied from 22 to 71% and 13 to 52% for phenanthrene and benz
o[a]pyrene, respectively. The proportions of contaminant solubilized w
ere inversely correlated with the sediments' organic carbon content. T
he extent of PAH solubilization among sediments by A. brasiliensis dig
estive Fluid was highly correlated with that of digestive fluid from t
he echiuran Urechis caupo and appears to be a consequence of surfactan
t properties of the fluids rather than of their enzymatic activity. Th
e proportion of PAHs solubilized in vitro was similar to in vivo measu
rements of solubilization for contaminant exposures lasting about 24 h
. However, with continued exposure, in vivo PAH concentrations in the
digestive fluid increased fivefold, suggesting that digestive fluid is
retained in the gut longer than sediment and thus accumulates PAHs th
rough sequential digestion of many gut volumes. This phenomenon may en
hance contaminant fugacity in the gut and increase the potential for b
ioaccumulation or toxicity.