COMPETITIVE-INHIBITION OF MOUSE-BRAIN NITRIC-OXIDE SYNTHASE BY AMILORIDE - A CASE FOR ENZYME CROSS-INHIBITION

Authors
ASCENZI P FEDERICO R MENEGATTI E VENTURINI G
Citation
P. Ascenzi et al., COMPETITIVE-INHIBITION OF MOUSE-BRAIN NITRIC-OXIDE SYNTHASE BY AMILORIDE - A CASE FOR ENZYME CROSS-INHIBITION, Biochemistry and molecular biology international, 43(3), 1997, pp. 507-511
Citations number
20
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
43
Issue
3
Year of publication
1997
Pages
507 - 511
Database
ISI
SICI code
1039-9712(1997)43:3<507:COMNSB>2.0.ZU;2-4
Abstract
Amiloride, a diuretic and antihypertensive drug, inhibits Na+ transpor ting systems, diamine oxidases and the human urinary plasminogen activ ator. Present results indicate that amiloride is a competitive inhibit or of mouse brain nitric oxide synthase (NOS; K-i = 4.5x10(-4) M, at p H 7.5 and 37.0 degrees C), but not a nitric oxide (NO) precursor, repr esenting a case for enzyme cross-inhibition. The decreased levels of N O, as a consequence of NOS inhibition, might induce some clinically-ad verse amiloride reactions, such as an unexpected reduced antihypertens ive effect.