DUAL TEMPERATURE MODEL FOR THE ESTIMATION OF ENERGETICS PARAMETERS FOR ACETYLCHOLINESTERASE INHIBITION BY CYCLOPHOSPHAMIDE

The present work addresses the 'dual temperature' model for the estima tion of Gibb's free energy change (Delta G), enthalpy change (Delta H) , heat of activation (Delta H) entropy change (Delta S), temperature coefficient (Q(10)) and activation energy (E-a) of chicken brain acety lcholinesterase (AChE) inhibited by cyclophosphamide monohydrate (CP). In this investigation, the PZ factor (number of sterically and energy wise favorable collisions occurring between CP and AChE) has also bee n studied. The inhibitor has considerably increased all energetics par ameters except Delta S and Q(10). These results are in general agreeme nt with the data from the other reported studies. The significance of the use of CP in cancer therapy and various aspects of thermodynamic p arameters have also been discussed.