ISOLATION AND POLYMORPHIC FREQUENCY DETECTION OF A NOVEL STR ON HUMAN-CHROMOSOME 14Q24.3

A single copy fragment (FD14-ca 1) containing 22 CA repetitive units w as isolated with (CA)(15) oligonucleotide probe from a human chromosom e 14q24.3 probe pool generated by microdissection, and proved to be a new short tandem repeat (STR) sequence through querying in GenBank of NCBI. The STR has 11 alleles in Chinese, and its polymorphic informati on content (PIC) is 0.85. Mendelian segregation was shown in 2 Chinese pedigrees with two generations. This STR has been accurately relocali zed on chromosome 14q24.3 by fluorescence in situ hybridization (FISH) . The accession numbers of this STR in GDB and GenBank database are D1 4S1435 and G31413 respectively. This STR would be able to be regarded as a novel genetic marker which can increase the genetic map accuracy in this chromosome region and improve the gene diagnosis on some gener ic diseases located in chromosome 14q24.3 band.