RAPID DNA QUANTIFICATION METHOD IN MICROPLATES USING DAUNORUBICINE FLUORESCENCE QUENCHING

Authors
GAGNE M PAGE M
Citation
M. Gagne et M. Page, RAPID DNA QUANTIFICATION METHOD IN MICROPLATES USING DAUNORUBICINE FLUORESCENCE QUENCHING, Oncology Reports, 5(3), 1998, pp. 653-655
Citations number
4
Categorie Soggetti
Oncology
Journal title
Oncology Reports → ACNP
ISSN journal
1021335X
Volume
5
Issue
3
Year of publication
1998
Pages
653 - 655
Database
ISI
SICI code
1021-335X(1998)5:3<653:RDQMIM>2.0.ZU;2-R
Abstract
We have developed a rapid, sensitive and inexpensive method to quantif y DNA in crude extracts or PCR reaction products using daunorubicine f luorescence quenching. We obtained a linear standard curve from 4.7 ng to 600 ng DNA and no interaction was observed in the presence of prot eins.