CLONING AND CHARACTERIZATION OF CDNAS CODING FOR HEAVY AND LIGHT-CHAINS OF AGGLUTINATING MONOCLONAL-ANTIBODY (HAG12ISLRH) SPECIFIC FOR HUMAN RED-BLOOD-CELLS

Complementary DNAs encoding the heavy and light chains of the Fab frag ment of mouse agglutinating monoclonal antibody against human red bloo d cells were cloned by polymerase chain reaction and their nucleotide sequences were determined. The sequence analysis showed that the varia ble regions of the heavy and light chains were the members of mouse he avy-chain subgroup IIa and kappa light-chain subgroup I, respectively. A few unusual amino acids in the constant regions of the heavy chain were also recognized. (C) 1998 Elsevier Science B.V.