Basic understanding of plant cell proliferation and differentiation is
imminent for applying modern techniques of genetic transformations. A
lthough remarkable progress has been made in the area of gene transfer
technology, little is known as to how plant cells differentiate in cu
ltures. Plant tissue culture has therefore remained an empirical scien
ce. In this review, an attempt has been made to assimilate the current
knowledge of the various biochemical and molecular parameters, which
play an important role in differentiation.