GENE-TRANSFER IN INDIAN MAJOR CARPS BY ELECTROPORATION

Authors
VENUGOPAL T PANDIAN TJ MATHAVAN S SARANGI N
Citation
T. Venugopal et al., GENE-TRANSFER IN INDIAN MAJOR CARPS BY ELECTROPORATION, Current Science, 74(7), 1998, pp. 636-638
Citations number
14
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Current Science → ACNP
ISSN journal
00113891
Volume
74
Issue
7
Year of publication
1998
Pages
636 - 638
Database
ISI
SICI code
0011-3891(1998)74:7<636:GIIMCB>2.0.ZU;2-#
Abstract
The rainbow trout growth hormone gene has been successfully transferre d into three species of Indian major carps rohu (Labeo rohita), mrigal (Cirrhinus mrigala) and catla (Catla catla) through electroporated sp erm cell. At 0.5 kV/cm (25 mu F capacitance, alpha resistance, and 2 p ulses) the gene transfer efficiency was 25% for rohu, 23% for mrigal a nd 13% for catla. This is the first report on gene transfer in Indian major carps.