RANDOMLY AMPLIFIED POLYMORPHIC DNA-BASED GENETIC-LINKAGE MAPS OF 3 APPLE CULTIVARS

Genetic linkage maps were created for three apple (Malas x domestica B orkh.) cultivars using data from two progenies ('Wijcik McIntosh' x NY 75431-67 and 'Wijcik Mcintosh' x NY75441-58). The maps consist primari ly of randomly amplified polymorphic DNA (RAPD) markers, but also cont ain six isozyme loci and four morphological markers (R-f, fruit skin c olor; V-f, scab resistance; Co, columnar growth habit; Ma, malic acid) . Maps were constructed using a double pseudo-testcross mapping format and JoinMap mapping software. An integrated 'Wijcik McIntosh' map was produced by combining marker data from both progenies into a single l inkage map. Homologous linkage groups from paternal maps were paired w ith their counterparts in the 'Wijcik McIntosh' map using locus bridge s composed of markers heterozygous in both parents of a progeny. The ' Wijcik McIntosh' map consists of 238 markers arranged in 19 linkage gr oups spanning 1206 cM. The NY 75441-67 map contains 110 markers in 16 linkage groups and the NY 75441-58 map consists of 183 markers in 18 l inkage groups. The average distance between markers in the maps was ap proximate to 5.0 cM.