PREPARATION OF IMMUNOLIPOSOMES DIRECTED AGAINST CD34 ANTIGEN AS TARGET

The My-10 monoclonal antibody has facilitated the search of haematopoi etic stem cells by recognizing selectively the human CD34 antigen. In the present work, My-10 immunoliposomes directed specifically against CD34+ cells were prepared, characterized and tested in vitro. Binding to target cells at 4 degrees C of immunoliposomes containing carboxyfl uorescein as aqueous marker was evaluated by flow cytometry and fluore scence microscopy. These immunoliposomes demonstrated their capacity t o bind specifically to CD34+ cells. Studies have shown that 9 antibodi es/vesicle were sufficient to obtain a good binding efficiency. The pr oduct was stable over one month at 4 degrees C in terms of leakage of encapsulated carboxyfluorescein, particle size and antigen binding cap acity. (C) 1998 Elsevier Science B.V.