A PHYSICAL MAP OF THE LEISHMANIA-MAJOR FRIEDLIN GENOME

Authors
IVENS AC LEWIS SM BAGHERZADEH A ZHANG L CHAN HM SMITH DF
Citation
Ac. Ivens et al., A PHYSICAL MAP OF THE LEISHMANIA-MAJOR FRIEDLIN GENOME, PCR methods and applications, 8(2), 1998, pp. 135-145
Citations number
45
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology,"Genetics & Heredity
Journal title
PCR methods and applications → ACNP
ISSN journal
10549803
Volume
8
Issue
2
Year of publication
1998
Pages
135 - 145
Database
ISI
SICI code
1054-9803(1998)8:2<135:APMOTL>2.0.ZU;2-U
Abstract
An extensive physical map of the Leishmania major Friedlin genome has been assembled by the combination of fingerprint analysis of a shuttle vector cosmid library and probe hybridization. The integrated data ob tained for 9004 fingerprinted clones and 974 probes have placed 91.2% of the 33.58-Mb genome into contigs representing each of the 36 chromo somes. This first-generation map has already provided a suitable frame work for both high-throughput DNA sequencing and functional studies of the L. major parasite.